This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human JAK2 antibody. JAK2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human JAK2 Antibody is added and binds to JAK2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated JAK2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human JAK2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.